Bercovich Z, Muskens J A
Department of Bacteriology, Institute for Animal Science and Health (ID-DLO), Lelystad, The Netherlands.
Vet J. 1999 Jan;157(1):61-7. doi: 10.1053/tvjl.1998.0252.
Eight-hundred-and-ninety-six cattle belonging to herds officially designated Brucella-free, and 190 cattle belonging to infected herds were tested with the skin delayed-type hypersensitivity (SDTH) test, using brucellin (273) prepared from a mucoid strain of Brucella abortus. An increase in skinfold thickness > or = 2 mm was considered a positive SDTH test. The serum agglutination test, complement fixation test and bacteriological examination were used to confirm SDTH test results. Results show that 6 of the 896 uninfected cattle tested positive in the SDTH test, indicating a 99.3% specificity. Of the 44 cattle that tested serologically or bacteriologically positive, 33 tested positive in the SDTH test, indicating a 75% sensitivity. The value of the SDTH test was demonstrated by its ability to detect infection earlier than serological tests, and by confirming infection in cattle with ambiguous serological test results. An increase in skinfold thickness > or = 1 mm in cattle in suspected herds should not be ignored, as it may indicate specific sensitization. We recommend the use of the SDTH test in combination with serological tests for early diagnosis of brucellosis in cattle.
对896头官方认定为无布鲁氏菌的牛群中的牛以及190头来自感染牛群的牛，使用由流产布鲁氏菌黏液样菌株制备的布鲁氏菌素（273）进行皮肤迟发型超敏反应（SDTH）检测。皮肤褶皱厚度增加≥2毫米被视为SDTH检测阳性。血清凝集试验、补体结合试验和细菌学检查用于确认SDTH检测结果。结果显示，896头未感染的牛中有6头在SDTH检测中呈阳性，特异性为99.3%。在44头血清学或细菌学检测呈阳性的牛中，33头在SDTH检测中呈阳性，敏感性为75%。SDTH检测的价值体现在其比血清学检测更早发现感染，以及在血清学检测结果不明确的牛中确认感染。疑似牛群中牛的皮肤褶皱厚度增加≥1毫米不应被忽视，因为这可能表明存在特异性致敏。我们建议将SDTH检测与血清学检测联合用于牛布鲁氏菌病的早期诊断。
