Morris I D
J Endocrinol. 1976 Dec;71(3):343-9. doi: 10.1677/joe.0.0710343.
The oestrogen specific, high-affinity cytosol receptor receptor (HAR) from amygdala, anterior, middle and posterior hypothalamus, pituitary and uterus was studied in the ovariectomized rat. A single in-vivo injection of oestradiol-17beta produced significant changes in both the tissue HAR concentrations and the apparent dissociation constants (Kd) determined in vitro. Four hours after oestradiol-17beta treatment (20 mug/kg), the HAR concentration was depleted in all tissues except the posterior hypothalamus. A lower dose of oestradiol-17beta (4 mug/kg) produced similar changes in HAR concentration with the exception of those in the amygdala and posterior hypothalamus. Twenty-four hours after oestradiol-17beta, HAR concentrations had returned to pre-injection levels in all tissues except the uterus. The uterine HAR concentrations were raised after both doses of oestradiol-17beta. The apparent tissue cytosol Kd values were decreased by both doses of oestradiol-17beta. The results suggest that brain, pituitary and uterine oestrogen cytosol HARs react to plasma oestrogen in a manner predictable by the steroid receptor hypothesis. The oestradiol-17beta-induced differential effects upon the tissue cytosol concentration may contribute to the overall spectrum of action of oestrogen in the central and peripheral reproductive processes.
在去卵巢大鼠中研究了来自杏仁核、下丘脑前、中、后部、垂体和子宫的雌激素特异性高亲和力胞质受体（HAR）。单次体内注射17β-雌二醇会使组织HAR浓度和体外测定的表观解离常数（Kd）均产生显著变化。在17β-雌二醇处理（20微克/千克）4小时后，除下丘脑后部外，所有组织中的HAR浓度均降低。较低剂量的17β-雌二醇（4微克/千克）在HAR浓度上产生了类似变化，但杏仁核和下丘脑后部除外。17β-雌二醇注射24小时后，除子宫外，所有组织中的HAR浓度均恢复到注射前水平。两种剂量的17β-雌二醇均使子宫HAR浓度升高。两种剂量的雌二醇均降低了表观组织胞质Kd值。结果表明，脑、垂体和子宫的雌激素胞质HARs对血浆雌激素的反应符合类固醇受体假说的预测。17β-雌二醇对组织胞质浓度的不同影响可能有助于雌激素在中枢和外周生殖过程中的整体作用谱。
