原肠胚形成过程中三个胚层的形成需要Rac1。

Rac1 is required for the formation of three germ layers during gastrulation.

作者信息

Sugihara K, Nakatsuji N, Nakamura K, Nakao K, Hashimoto R, Otani H, Sakagami H, Kondo H, Nozawa S, Aiba A, Katsuki M

机构信息

Division of DNA Biology and Embryo Engineering, Center for Experimental Medicine, The Institute of Medical Science, University of Tokyo, Japan.

出版信息

Oncogene. 1998 Dec 31;17(26):3427-33. doi: 10.1038/sj.onc.1202595.

DOI:10.1038/sj.onc.1202595

PMID:10030666

Abstract

The Rac1, a member of the Rho family proteins, regulates actin organization of cytoskeleton and cell adhesion. We used genetic analysis to elucidate the role of Rac1 in mouse embryonic development. The rac1 deficient embryos showed numerous cell deaths in the space between the embryonic ectoderm and endoderm at the primitive streak stage. Investigation of the primary epiblast culture isolated from rac1 deficient embryos indicated that Rac1 is involved in lamellipodia formation, cell adhesion and cell migration in vivo. These results suggest that Rac1-mediated cell adhesion is essential for the formation of three germ layers during gastrulation.

摘要

Rac1是Rho家族蛋白的成员之一，可调节细胞骨架的肌动蛋白组织和细胞黏附。我们使用基因分析来阐明Rac1在小鼠胚胎发育中的作用。rac1基因缺陷的胚胎在原条期胚胎外胚层和内胚层之间的空间出现大量细胞死亡。对从rac1基因缺陷胚胎中分离出的原代上胚层培养物的研究表明，Rac1在体内参与板状伪足的形成、细胞黏附和细胞迁移。这些结果表明，Rac1介导的细胞黏附对于原肠胚形成过程中三个胚层的形成至关重要。