Lawson A J, Shafi M S, Pathak K, Stanley J
Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, Colindale, London, UK.
Epidemiol Infect. 1998 Dec;121(3):547-53. doi: 10.1017/s0950268898001630.
The prevalence of campylobacter gastroenteritis has been estimated by bacterial isolation using selective culture. However, there is evidence that certain species and strains are not recovered on selective agars. We have therefore compared direct PCR assays of faecal samples with campylobacter culture, and explored the potential of PCR for simultaneous detection and identification to the species level. Two hundred unselected faecal samples from cases of acute gastroenteritis were cultured on modified charcoal cefoperazone deoxycholate agar and subjected to DNA extraction and PCR assay. Culture on CCDA indicated that 16 of the 200 samples contained 'Campylobacter spp.'. By contrast, PCR assays detected campylobacters in 19 of the 200 samples, including 15 of the culture-positive samples, and further identified them as: C. jejuni (16), C. coli (2) and C. hyointestinalis (1). These results show that PCR offers a different perspective on the incidence and identity of campylobacters in human gastroenteritis.
空肠弯曲菌肠胃炎的患病率已通过使用选择性培养的细菌分离法进行了估算。然而，有证据表明某些菌种和菌株无法在选择性琼脂上生长。因此，我们比较了粪便样本的直接聚合酶链反应（PCR）检测与弯曲菌培养，并探讨了PCR在同时检测和鉴定到菌种水平方面的潜力。对200例急性肠胃炎病例的未经筛选的粪便样本在改良的炭头孢哌酮脱氧胆酸盐琼脂上进行培养，并进行DNA提取和PCR检测。在CCDA上的培养表明，200个样本中有16个含有“弯曲菌属”。相比之下，PCR检测在200个样本中的19个样本中检测到弯曲菌，其中包括15个培养阳性样本，并进一步将它们鉴定为：空肠弯曲菌（16个）、结肠弯曲菌（2个）和猪肠弯曲菌（1个）。这些结果表明，PCR为人类肠胃炎中弯曲菌的发病率和鉴定提供了不同的视角。
