Kern D H, Fritze D, Drogemuller C R, Pilch Y H
J Natl Cancer Inst. 1976 Jul;57(1):97-103. doi: 10.1093/jnci/57.1.97.
Xenogeneic immune RNA (I-RNA), extracted from the lymphoid organs of sheep or guinea pigs immunized with human tumor cells, mediated in vitro cytotoxic immune responses that were directed specifically against tumor-associated antigens of human tumor target cells. Normal human peripheral blood lymphocytes from healthy donors became markedly more cytotoxic for human tumor target cells after being incubated with I-RNA extracted from the lymphoid organs of animals that had been immunized with that particular tumor. Gastric carcinoma, malignant melanoma, and carcinoma of the breast were studied. Lymphocytes incubated with RNA from animals immunized with only complete Freund's adjuvant evidenced no increased cytotoxic activity. RNA extracted from the lymphoid organs of animals immunized with normal skin fibroblasts that were autologous to the immunizing tumor, when incubated with normal allogeneic lymphocytes, also mediated cytotoxic immune reactions against tumor target cells. These immune responses probably were directed principally against normal transplantation antigens. However, when lymphocytes that were autologous to the immunizing tumor and/or the tumor target cells were incubated with RNA from animals immunized with autologous normal fibroblasts, cytotoxicity did not increase. Only I-RNA extracted from donor animals specifically immunized with tumor cells mediated cytotoxic antitumor immune responses when incubated with autologous lymphocytes.
异种免疫RNA（I-RNA），从用人类肿瘤细胞免疫的绵羊或豚鼠的淋巴器官中提取，介导了体外细胞毒性免疫反应，该反应特异性地针对人类肿瘤靶细胞的肿瘤相关抗原。来自健康供体的正常人外周血淋巴细胞在用从用该特定肿瘤免疫的动物的淋巴器官中提取的I-RNA孵育后，对人类肿瘤靶细胞的细胞毒性明显增强。研究了胃癌、恶性黑色素瘤和乳腺癌。用仅含完全弗氏佐剂免疫的动物的RNA孵育的淋巴细胞未显示出细胞毒性活性增加。从与免疫肿瘤自体的正常皮肤成纤维细胞免疫的动物的淋巴器官中提取的RNA，与正常同种异体淋巴细胞孵育时，也介导了针对肿瘤靶细胞的细胞毒性免疫反应。这些免疫反应可能主要针对正常移植抗原。然而，当与免疫肿瘤和/或肿瘤靶细胞自体的淋巴细胞与用自体正常成纤维细胞免疫的动物的RNA孵育时，细胞毒性并未增加。只有从用肿瘤细胞特异性免疫的供体动物中提取的I-RNA与自体淋巴细胞孵育时，才介导细胞毒性抗肿瘤免疫反应。
