Ageitos A G, Varney M L, Bierman P J, Vose J M, Warkentin P I, Talmadge J E
Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68198-5660, USA.
Bone Marrow Transplant. 1999 Jan;23(1):63-9. doi: 10.1038/sj.bmt.1701524.
This study compares the immune properties of peripheral blood stem cell (PSC) products mobilized with different hematopoietic growth factors (HGFs) as well as apheresis products and peripheral blood leukocytes (PBL) from normal individuals. We found that monocytes in mobilized PSC products appear to inhibit T cell function independent of whether granulocyte colony-stimulating factor (G-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF) was used for mobilization. In addition, the GF used to mobilize the stem cell product may be less important to the CD4:CD8 ratio than the extent of prior chemotherapy, as we found an inverse correlation between chemotherapy and the CD4:CD8 ratio. In other observations, all apheresis products, whether mobilized or unmobilized, contained significantly more monocytes compared to normal PBL. The mononuclear cells (MNC) from G-CSF or GM-CSF mobilized PSC products had a similar T cell phytohemagglutinin (PHA) mitogenic response that was significantly lower (P = 0.001 and P = 0.005, respectively) than non-mobilized apheresis products. We also examined the T cell inhibitor (TI) activity of the MNC from the PSC products for allogeneic lymphocyte proliferation and found that PSC products significantly reduced the proliferation of allogeneic PBL to PHA. A significant correlation (P = 0.001, r = 0.517) between the frequency of monocytes and TI activity also was observed.
本研究比较了用不同造血生长因子（HGFs）动员的外周血干细胞（PSC）产品以及单采产品和正常个体外周血白细胞（PBL）的免疫特性。我们发现，动员的PSC产品中的单核细胞似乎抑制T细胞功能，无论使用粒细胞集落刺激因子（G-CSF）还是粒细胞-巨噬细胞集落刺激因子（GM-CSF）进行动员。此外，用于动员干细胞产品的生长因子对CD4:CD8比值的重要性可能不如先前化疗的程度，因为我们发现化疗与CD4:CD8比值呈负相关。在其他观察中，所有单采产品，无论是否动员，与正常PBL相比，单核细胞含量均显著更高。来自G-CSF或GM-CSF动员的PSC产品的单核细胞（MNC）具有相似的T细胞植物血凝素（PHA）促有丝分裂反应，该反应显著低于未动员的单采产品（分别为P = 0.001和P = 0.005）。我们还检查了PSC产品中MNC对同种异体淋巴细胞增殖的T细胞抑制剂（TI）活性，发现PSC产品显著降低了同种异体PBL对PHA的增殖。还观察到单核细胞频率与TI活性之间存在显著相关性（P = 0.001，r = 0.517）。
