Delgado M D, Chernukhin I V, Bigas A, Klenova E M, León J
Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria, Santander, Spain.
FEBS Lett. 1999 Feb 5;444(1):5-10. doi: 10.1016/s0014-5793(99)00013-7.
CTCF is a transcriptional repressor of the c-myc gene. Although CTCF has been characterized in some detail, there is very little information about the regulation of CTCF activity. Therefore we investigated CTCF expression and phosphorylation during induced differentiation of human myeloid leukemia cells. We found that: (i) both CTCF mRNA and protein are down-regulated during terminal differentiation in most cell lines tested; (ii) CTCF down-regulation is retarded and less pronounced than that of c-myc; (iii) CTCF protein is differentially phosphorylated and the phosphorylation profiles depend on the differentiation pathway. We concluded that CTCF expression and activity is controlled at transcriptional and post-transcriptional levels.
CTCF是c-myc基因的转录抑制因子。尽管CTCF已得到一定程度的详细表征，但关于CTCF活性调控的信息却非常少。因此，我们研究了人髓系白血病细胞诱导分化过程中CTCF的表达和磷酸化情况。我们发现：（i）在大多数测试的细胞系中，终末分化过程中CTCF的mRNA和蛋白质均下调；（ii）CTCF的下调比c-myc的下调延迟且不那么明显；（iii）CTCF蛋白存在差异磷酸化，且磷酸化谱取决于分化途径。我们得出结论，CTCF的表达和活性在转录和转录后水平受到调控。
