Todosiĭchuk S R, Isakova D M, Kvasnikov E I
Mikrobiologiia. 1976 May-Jun;45:433-6.
The effect of biotin on the specific growth rate of Candida tropicalis was studied on media with various sources of carbon nutrition at temperatures of 29 and 39degreesC. The yeast was found to require 3--5 times less biotin on the medium with paraffin than on the medium with glucose and hexodecane, and 3--4 times more biotin on the same substrate with an increase in temperature. The growth rate increased by 36--40% on liquid paraffins containing 0.5 mcg/litre of biotin at 29degreesC and 1.5--2.0 mcg/litre of biotin at 39degreesC. The saturation constant of biotin increased from 0.033 to 0.063 mcg/litre on the medium with paraffin, and more by an order on the medium with glucose and hexadecane, at a rise of growth temperature from 29 to 39degreesC.
在29摄氏度和39摄氏度的温度下，研究了生物素对热带假丝酵母在含有不同碳源营养的培养基上比生长速率的影响。结果发现，酵母在以石蜡为培养基时所需的生物素比以葡萄糖和十六烷为培养基时少3至5倍，而在相同底物上，随着温度升高，所需生物素多3至4倍。在29摄氏度下，含有0.5微克/升生物素的液体石蜡上生长速率提高了36%至40%，在39摄氏度下，含有1.5至2.0微克/升生物素的液体石蜡上生长速率提高了36%至40%。随着生长温度从29摄氏度升至39摄氏度，以石蜡为培养基时生物素的饱和常数从0.033微克/升增加到0.063微克/升，以葡萄糖和十六烷为培养基时则增加了一个数量级以上。
