Zaretskaia A N
Mikrobiologiia. 1976 Sep-Oct;45(5):873-8.
Transformation of lucerne nodule bacteria was tried as a method for increasing their activity. About 100 strains resistant to 1000 units/ml of streptomycin have been obtained by transformation; 34 strains were tested for the activity of nitrogen fixation in conditions of a microvegetative experiment according to the scheme: strain-donor, strain-recipient, transformed strains. The activity varied within a wide range: +/-30--40% cf. the activity of the parent strains. The transformed strains were passaged through plants and those with a still higher activity were selected. This evidence confirms the conception of Natman concerning genetic compatibility of plants and nodule bacteria when those bacterial forms whose genome corresponds to the plant genome are selected by the macrosymbiont.
尝试将苜蓿根瘤菌转化作为提高其活性的一种方法。通过转化已获得约100株对1000单位/毫升链霉素具有抗性的菌株；根据以下方案在微营养实验条件下对34株菌株进行了固氮活性测试：供体菌株、受体菌株、转化菌株。活性变化范围很广：与亲本菌株的活性相比为+/-30--40%。将转化菌株接种到植物上，并筛选出活性更高的菌株。这一证据证实了纳特曼关于植物与根瘤菌遗传相容性的概念，即当大共生体选择其基因组与植物基因组相对应的细菌形态时。
