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对体外培养的哺乳动物细胞紫外线照射后切除修复研究的贡献。

Contribution to the study of excision repair following UV irradiation in mammalian cells cultivated in vitro.

作者信息

Hochmann J, Masek F, Chalupa I

出版信息

Neoplasma. 1976;23(6):623-8.

PMID:1004659
Abstract

The absence of pyrimidine dimer excision after UV irradiation in rat cell line was confirmed. The possibility of excision repair system depression by acidification of medium with H3PO4 in HeLa cells has been proved. The results obtained by two different methods of deproteinization and DNA isolation are in good conformity.

摘要

已证实在大鼠细胞系中紫外线照射后嘧啶二聚体切除缺失。已证明在HeLa细胞中用H3PO4酸化培养基会导致切除修复系统抑制。通过两种不同的脱蛋白和DNA分离方法获得的结果高度一致。

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