Purification and characterization of a multienzymatic complex in sugarcane, a C4 plant. - Suppr | 超能文献

Purification and characterization of a multienzymatic complex in sugarcane, a C4 plant.

作者信息

Baret P, Cesari M, Queiroz C, Rouch C, Meunier J C, Cadet F

机构信息

Laboratoire de biochimie et génétique moléculaire, faculté des sciences et technologies, université de la Réunion, Saint-Denis Messag, France.

出版信息

C R Acad Sci III. 1999 Jan;322(1):29-34. doi: 10.1016/s0764-4469(99)80014-6.

DOI:10.1016/s0764-4469(99)80014-6

Abstract

We have discovered a multienzymatic complex in fresh young sugarcane leaves. This complex is constituted of three enzymes: PEPcase, NADP-MDH and malic enzyme. After successive molecular sieving chromatography, we have obtained a highly purified sample of the complex which has a molecular weight of 711 kDa. Its functional interest has been evaluated by comparing the kinetic properties of the enzymes in their free forms to those in their complexed form. We show that the association of the three enzymes leads to important changes in their respective kinetic properties.

摘要

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