Singh J, McLean J A, Pritchard D E, Montaser A, Patierno S R
Department of Pharmacology, George Washington University, Washington, DC 20052, USA.
Toxicol Sci. 1998 Dec;46(2):260-5. doi: 10.1006/toxs.1998.2512.
A novel method is described for the sensitive detection of chromium-DNA adducts. Chromium-DNA adducts were determined in 1 microgram of DNA from normal human lung fibroblasts exposed to sodium chromate using microscale flow injection analysis with a direct injection high-efficiency nebulizer and inductively coupled plasma mass spectrometry detection. The frequency of Cr-DNA adducts increased in a dose-dependent sigmoidal manner, indicating saturation and toxicity. The low detection limits (on the order of parts per trillion) allows the detection of as few as two Cr adducts per 10,000 bases, which, coupled with the small DNA sample requirement, makes this technique suitable for measuring metal-DNA adducts as biomarkers of exposure to toxic and carcinogenic metals such as Cr, in cultured cells, animals, and humans.
本文描述了一种用于灵敏检测铬 - DNA加合物的新方法。使用带直接进样高效雾化器的微量流动注射分析和电感耦合等离子体质谱检测，对暴露于铬酸钠的正常人肺成纤维细胞中1微克DNA中的铬 - DNA加合物进行了测定。铬 - DNA加合物的频率以剂量依赖性的S形方式增加，表明存在饱和及毒性。低检测限（万亿分之一量级）使得每10,000个碱基中低至两个铬加合物即可被检测到，再加上对DNA样品需求量小，使得该技术适用于测量金属 - DNA加合物，作为暴露于诸如铬等有毒和致癌金属的生物标志物，可用于培养细胞、动物及人类。
