Jordan H L, Liang Y, Hudson L C, Tompkins W A
Department of Microbiology, Pathology, and Parasitology, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
Am J Vet Res. 1999 Feb;60(2):211-5.
To examine shedding of cell-free and cell-associated feline immunodeficiency virus (FIV) in semen of domestic cats during acute infection.
7 specific-pathogen-free sexually intact male cats.
6 cats were inoculated IV with 5 x 10(6) 50% tissue culture infective doses of FIV-NCSU1, and 1 cat served as an uninfected (control) cat. Infection was confirmed in the 6 cats. Periodically for up to 16 weeks after inoculation, cats were anesthetized and ejaculates obtained by use of electroejaculation. Virus was isolated from filtered seminal plasma and washed seminal cells by co-cultivation with a feline CD4+ T-cell line. Seminal cell lysates were also examined for a 582-base pair segment of FIV gag provirus DNA, using a nested polymerase chain reaction amplification.
During the acute phase of FIV infection, virus was evident in semen of 5 inoculated cats. Five cats had virus-positive seminal plasma and 3 had virus-positive cellular constituents during the study. Virus was isolated from 8/22 (36%) seminal plasma samples and 2/17 (18%) seminal cell specimens. Provirus DNA was detected in 5/24 (21%) seminal cell lysates. Cell-free virus was isolated as early as 6 weeks after inoculation, whereas cell-associated virus was isolated as early as 12 weeks after inoculation. Provirus DNA was detected in seminal cells from one cat as early as 1 week after inoculation.
Cell-free and cell-associated FIV are shed in semen of cats early during the course of infection. Samples obtained before seroconversion may contain virus. Virus shedding in ejaculates varies between and within cats during acute infection.
检测家猫急性感染期间精液中游离型和细胞相关型猫免疫缺陷病毒（FIV）的排出情况。
7只无特定病原体、性成熟的雄性猫。
6只猫经静脉接种5×10(6) 50%组织培养感染剂量的FIV-NCSU1，1只猫作为未感染（对照）猫。确认6只猫被感染。接种后长达16周内定期对猫进行麻醉，并通过电刺激采精获取精液。通过与猫CD4+ T细胞系共培养，从过滤后的精浆和洗涤后的精细胞中分离病毒。还使用巢式聚合酶链反应扩增检测精细胞裂解物中FIV gag前病毒DNA的582碱基对片段。
在FIV感染急性期，5只接种猫的精液中可检测到病毒。研究期间，5只猫的精浆病毒呈阳性，3只猫的细胞成分病毒呈阳性。从22份精浆样本中的8份（36%）和17份精细胞标本中的2份（18%）分离出病毒。在24份精细胞裂解物中的5份（21%）检测到前病毒DNA。接种后最早6周分离出游离型病毒，而接种后最早12周分离出细胞相关型病毒。接种后最早1周在1只猫的精细胞中检测到前病毒DNA。
在感染过程早期，猫精液中会排出游离型和细胞相关型FIV。血清转化前采集的样本可能含有病毒。急性感染期间，不同猫之间以及同一只猫体内射精液中的病毒排出情况存在差异。
