Waite E R, Collins M J, van Duin A C
Fossil Fuels and Environmental Geochemistry (NRG), University of Newcastle upon Tyne, UK.
Int J Legal Med. 1999;112(2):124-31. doi: 10.1007/s004140050214.
The proportion of D- to L-enantiomers of aspartic acid in metabolically isolated proteins has been used by forensic scientists to estimate age at death. We have demonstrated the interference of a derivative of hydroxyproline (N-TFA isopropyl Hyp ester) with the N-TFA isopropyl L-Aspartic (Asp) acid ester during gas chromatography of amino acids. This has serious implications for the accurate quantification of the D- to L-Asp ratio extracted from collagenous proteins. Having demonstrated the potential for this co-elution in amino acid standards, acid-soluble dentine proteins and non-mineralised collagen, we argue that this problem can be overcome either by high resolution separation or by analysis of the (Hyp-poor) non-collagenous protein fraction.
法医科学家利用代谢分离蛋白质中天冬氨酸D型与L型对映体的比例来估计死亡年龄。我们已经证明，在氨基酸气相色谱分析过程中，羟脯氨酸衍生物（N - TFA异丙基羟脯氨酸酯）会干扰N - TFA异丙基L - 天冬氨酸（天冬氨酸）酯。这对从胶原蛋白中提取的D型与L型天冬氨酸比例的准确定量具有严重影响。在氨基酸标准品、酸溶性牙本质蛋白和非矿化胶原蛋白中证实了这种共洗脱的可能性后，我们认为可以通过高分辨率分离或分析（低羟脯氨酸）非胶原蛋白部分来克服这个问题。
