犬白细胞介素-18和犬白细胞介素-1β转换酶cDNA的克隆及犬白细胞介素-18的表达

Cloning of cDNA for canine interleukin-18 and canine interleukin-1beta converting enzyme and expression of canine interleukin-18.

作者信息

Okano F, Satoh M, Ido T, Yamada K

机构信息

Toray Industries, Inc., Chemicals Research Laboratories, Nagoya, Japan.

出版信息

J Interferon Cytokine Res. 1999 Jan;19(1):27-32. doi: 10.1089/107999099314388.

DOI:10.1089/107999099314388

PMID:10048765

Abstract

Cloning of canine interleukin-18 (IL-18) and canine interleukin-1beta converting enzyme (ICE) cDNA was carried out by using murine IL-18 cDNA and human ICE cDNA, respectively, as probes. Sequence homology to known sequences of human, mouse, or rat genes was noted at nucleotide and amino acid levels. Canine IL-18 mRNA was expressed in various canine organs, whereas canine ICE mRNA was expressed in only a few, particularly in the brain and testis. Cloned canine IL-18 cDNA was expressed in Escherichia coli. The resulting protein promoted induction of canine interferon-y (IFN-y) from stimulated canine lymphocytes. Canine IL-18 and canine IL-12 produced canine IFN-gamma synergistically. Canine IL-18 suppressed the growth of tumor cells transplanted in SCID mice. Cloned canine IL-18 should prove useful as an anticancer agent.

摘要

分别以鼠白细胞介素-18（IL-18）cDNA和人白细胞介素-1β转换酶（ICE）cDNA为探针，进行犬IL-18和犬ICE cDNA的克隆。在核苷酸和氨基酸水平上，发现其与人类、小鼠或大鼠基因的已知序列具有序列同源性。犬IL-18 mRNA在犬的各种器官中表达，而犬ICE mRNA仅在少数器官中表达，特别是在脑和睾丸中。克隆的犬IL-18 cDNA在大肠杆菌中表达。产生的蛋白质促进了受刺激的犬淋巴细胞产生犬干扰素-γ（IFN-γ）。犬IL-18和犬IL-12协同产生犬干扰素-γ。犬IL-18抑制了移植到严重联合免疫缺陷（SCID）小鼠体内的肿瘤细胞的生长。克隆的犬IL-18应被证明可作为一种抗癌剂。