Peng H J, Tsai J J, Chang Z N, Shen H D, Tsai L C, Su S N
Department of Medical Research, Veterans General Hospital-Taipei, Taiwan, ROC.
Int Arch Allergy Immunol. 1998 Dec;117(4):224-30. doi: 10.1159/000024015.
The effects of denaturation of ovalbumin (OVA) on the induction of oral sensitization in guinea pigs were examined.
Guinea pig antibody and airway responses were assessed after 10 feedings of chemically or heat-denatured OVA or egg white (EW).
Their specific IgG, IgG1 and IgG2 antibody responses were orally sensitized by OVA or EW, but not by chemically or heat-denatured OVA or EW. When further exposed to 0.1% OVA or conalbumin aerosol, those fed OVA or EW, but not denatured OVA or EW, had increased pulmonary resistance and decreased tidal volume. On the other hand, in those fed denatured OVA, boiled EW or saline only, a second sensitization with 1% OVA aerosol generated antibody responses and airway hyperreactivity. Using a sandwich ELISA, guinea pig serum OVA was detected after feeding EW, but not chemically denatured or boiled EW.
It is likely that guinea pig gut absorption of OVA may result in oral sensitization. Chemical or heat denaturation of proteins may minimize their intestinal uptake and thus abrogates the induction of oral sensitization in guinea pigs.
研究了卵清蛋白（OVA）变性对豚鼠口服致敏诱导的影响。
在对豚鼠喂食化学或热变性的OVA或蛋清（EW）10次后，评估其抗体和气道反应。
它们的特异性IgG、IgG1和IgG2抗体反应通过OVA或EW口服致敏，但不通过化学或热变性的OVA或EW致敏。当进一步暴露于0.1%的OVA或伴清蛋白气雾剂时，那些喂食OVA或EW而非变性OVA或EW的豚鼠，肺阻力增加，潮气量减少。另一方面，在那些仅喂食变性OVA、煮沸的EW或生理盐水的豚鼠中，用1%的OVA气雾剂进行二次致敏会产生抗体反应和气道高反应性。使用夹心ELISA法，喂食EW后可检测到豚鼠血清中的OVA，但化学变性或煮沸的EW后则检测不到。
豚鼠肠道吸收OVA可能导致口服致敏。蛋白质的化学或热变性可能会使其肠道摄取量降至最低，从而消除豚鼠口服致敏的诱导。
