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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Gamo S, Dodo K, Matakatsu H, Tanaka Y

Department of Life Sciences, Osaka Prefecture University, Sakai, Japan.

Toxicol Lett. 1998 Nov 23;100-101:329-37. doi: 10.1016/s0378-4274(98)00203-3.

For a genetical approach to the molecular mechanisms of general anesthesia, we isolated 14 Drosophila transposon tagging mutants which showed altered sensitivities to diethylether anesthesia. Two mutated genes were identified and analyzed for their structures and functions. 2. One sensitive mutant, para(hd838), has a transposon, P-element, insertion in the 2nd intron of para locus (14C) which encodes an alpha subunit of a sodium channel. 3. Another sensitive mutant, eth(as)311. has a P-element in the 5'-untranslated region of Drosophila calreticulin gene (86BC). Calreticulin is known as a multifunctional calcium-binding protein in the endoplasmic reticulum of non-muscle cells. 4. We obtained their revertants to show that the P-element insertion in each gene induced the hypersensitivity to ether anesthesia. We describe here that the sodium channel and calreticulin might be targets for the ether anesthesia.

为了从遗传学角度研究全身麻醉的分子机制，我们分离出14个对乙醚麻醉敏感性发生改变的果蝇转座子标签突变体。鉴定了两个突变基因，并对其结构和功能进行了分析。2. 一个敏感突变体para(hd838)，其转座子P因子插入到para基因座（14C）的第2个内含子中，该基因座编码钠通道的一个α亚基。3. 另一个敏感突变体eth(as)311，其P因子位于果蝇钙网蛋白基因（86BC）的5'非翻译区。钙网蛋白是一种已知的非肌肉细胞内质网中的多功能钙结合蛋白。4. 我们获得了它们的回复体，以表明每个基因中的P因子插入导致了对乙醚麻醉的超敏反应。我们在此描述，钠通道和钙网蛋白可能是乙醚麻醉的靶点。

Gamo S, Dodo K, Matakatsu H, Tanaka Y

Department of Life Sciences, Osaka Prefecture University, Sakai, Japan.

Toxicol Lett. 1998 Nov 23;100-101:329-37. doi: 10.1016/s0378-4274(98)00203-3.

For a genetical approach to the molecular mechanisms of general anesthesia, we isolated 14 Drosophila transposon tagging mutants which showed altered sensitivities to diethylether anesthesia. Two mutated genes were identified and analyzed for their structures and functions. 2. One sensitive mutant, para(hd838), has a transposon, P-element, insertion in the 2nd intron of para locus (14C) which encodes an alpha subunit of a sodium channel. 3. Another sensitive mutant, eth(as)311. has a P-element in the 5'-untranslated region of Drosophila calreticulin gene (86BC). Calreticulin is known as a multifunctional calcium-binding protein in the endoplasmic reticulum of non-muscle cells. 4. We obtained their revertants to show that the P-element insertion in each gene induced the hypersensitivity to ether anesthesia. We describe here that the sodium channel and calreticulin might be targets for the ether anesthesia.

为了从遗传学角度研究全身麻醉的分子机制，我们分离出14个对乙醚麻醉敏感性发生改变的果蝇转座子标签突变体。鉴定了两个突变基因，并对其结构和功能进行了分析。2. 一个敏感突变体para(hd838)，其转座子P因子插入到para基因座（14C）的第2个内含子中，该基因座编码钠通道的一个α亚基。3. 另一个敏感突变体eth(as)311，其P因子位于果蝇钙网蛋白基因（86BC）的5'非翻译区。钙网蛋白是一种已知的非肌肉细胞内质网中的多功能钙结合蛋白。4. 我们获得了它们的回复体，以表明每个基因中的P因子插入导致了对乙醚麻醉的超敏反应。我们在此描述，钠通道和钙网蛋白可能是乙醚麻醉的靶点。