Hisatomi O, Honkawa H, Imanishi Y, Satoh T, Tokunaga F
Graduate School of Science, Osaka University, Japan.
Biochem Biophys Res Commun. 1999 Feb 16;255(2):216-20. doi: 10.1006/bbrc.1999.0165.
Three kinds of cDNAs encoding the putative photoreceptor-specific guanylate cyclases (GCs), OlGC-R1, OlGC-R2, and OlGC-C, were isolated from a retinal cDNA library of the medaka, Oryzias latipes. The deduced amino acid sequences of OlGC-R1 and -C are closely related but slightly different from those of OlGC4 and OlGC5, respectively. In situ hybridization locates the mRNA of both OlGC-R1 and -R2 in rods, and OlGC-C mRNA is found in all four types of cone cells. It is likely that medaka rods and cones produce distinct GC subtypes and that two kinds of photoreceptor-specific GCs are coexpressed in rods. Also, hybridization signals are detected in the pineal organ, suggesting that OlGC-R1 and -C contribute also to phototransduction in pinealocytes.
从青鳉（Oryzias latipes）的视网膜cDNA文库中分离出三种编码假定的光感受器特异性鸟苷酸环化酶（GCs）的cDNA，即OlGC-R1、OlGC-R2和OlGC-C。OlGC-R1和OlGC-C推导的氨基酸序列密切相关，但分别与OlGC4和OlGC5的序列略有不同。原位杂交将OlGC-R1和OlGC-R2的mRNA定位在视杆细胞中，而OlGC-C mRNA在所有四种类型的视锥细胞中均有发现。青鳉的视杆细胞和视锥细胞可能产生不同的GC亚型，并且两种光感受器特异性GCs在视杆细胞中共表达。此外，在松果体器官中检测到杂交信号，表明OlGC-R1和OlGC-C也参与松果体细胞的光转导。
