Tan Y J, Beerheide W, Ting A E
Institute of Molecular and Cell Biology, Singapore.
Biochem Biophys Res Commun. 1999 Feb 16;255(2):334-9. doi: 10.1006/bbrc.1999.0222.
The overexpression of Bax, a member of the Bcl-2 family, promotes cell death and the dimerization (or oligomerization) of Bax has been shown to be important for its function. Using size-exclusion chromatography and in vitro cross-linking experiments, we demonstrated that Bax exists mainly as a large oligomer of approximately 30 monomeric units. Furthermore, several binding assays demonstrated that Bcl-XL, an anti-apoptotic member of the Bcl-2 family, can bind to the oligomeric form of Bax without requiring Bax to dissociate to monomers.
Bcl-2家族成员Bax的过表达会促进细胞死亡，并且已证明Bax的二聚化（或寡聚化）对其功能很重要。通过尺寸排阻色谱法和体外交联实验，我们证明Bax主要以大约30个单体单元的大寡聚体形式存在。此外，多项结合试验表明，Bcl-2家族的抗凋亡成员Bcl-XL可以结合Bax的寡聚形式，而无需Bax解离成单体。
