通过体细胞杂交分析对猪的钠钾ATP酶α2亚基（ATP1A2）基因和肌肉磷酸果糖激酶（PFKM）基因进行定位。

Mapping of the Na+, K(+)-ATPase subunit alpha 2 (ATP1A2) and muscle phosphofructokinase (PFKM) genes in pig by somatic cell hybrid analysis.

作者信息

Fontanesi L, Davoli R, Zijlstra C, Bosma A A, Russo V

机构信息

DIPROVAL-Sezione di Allevamenti Zootecnici, Faculty of Agriculture, University of Bologna, Reggio Emilia, Italy.

出版信息

Anim Genet. 1999 Feb;30(1):57-9. doi: 10.1046/j.1365-2052.1999.00407.x.

DOI:10.1046/j.1365-2052.1999.00407.x

PMID:10050286

Abstract

Two expressed sequence tags were isolated from a porcine skeletal muscle cDNA library and identified as the putative partial cDNAs of the porcine Na+, K(+)-ATPase subunit alpha 2 (ATP1A2) and muscle phosphofructokinase (PFKM) genes after sequencing and homology search. Results of analysis of a pig-rodent somatic cell hybrid panel by PCR allowed the assignments of ATP1A2 to porcine chromosome (chr) 4 and of PFKM to porcine chr 5. These assignments support previously observed conservation of syntenic relationships between human chr 1 and porcine chr 4 and between human chr 12 and porcine chr 5.

摘要

从猪骨骼肌cDNA文库中分离出两个表达序列标签，经测序和同源性搜索后，鉴定为猪Na +, K(+)-ATP酶α2亚基（ATP1A2）和肌肉磷酸果糖激酶（PFKM）基因的假定部分cDNA。通过PCR对猪-啮齿动物体细胞杂种面板进行分析的结果，将ATP1A2定位到猪染色体（chr）4，将PFKM定位到猪chr 5。这些定位结果支持了先前观察到的人类chr 1与猪chr 4之间以及人类chr 12与猪chr 5之间同线性关系的保守性。