Wang I Y, Cornillie F J, Barsamian S, Street D, Manconi F, Fraser I S
Sydney Centre for Reproductive Health Research, Department of Obstetrics & Gynaecology, University of Sydney, NSW, Australia.
Mol Hum Reprod. 1999 Jan;5(1):79-83. doi: 10.1093/molehr/5.1.79.
The objective of this study was to evaluate the possible role of four lysosomal enzymes in endometrial function and remodelling during the normal menstrual cycle by fluorimetric measurement (acid phosphatase, N-acetyl-beta-D-glucosaminidase, alpha-L-fucosidase and alpha-D-mannosidase). A prospective study was conducted of 45 endometrial biopsies obtained from women with normal menstrual cycles. Activity of all four enzymes was identified in human endometrium. Activity of acid phosphatase and N-acetyl-beta-D-glucosaminidase was relatively high, whilst that of alpha-L-fucosidase and alpha-D-mannosidase was low. There was no significant change in the activity of any of the four enzymes from the proliferative to the secretory phase of the cycle. This study suggests that the activity of these enzymes remains constant throughout a major portion of the normal cycle.
本研究的目的是通过荧光测量法（酸性磷酸酶、N-乙酰-β-D-氨基葡萄糖苷酶、α-L-岩藻糖苷酶和α-D-甘露糖苷酶）评估四种溶酶体酶在正常月经周期中子宫内膜功能和重塑过程中的可能作用。对45例月经周期正常的女性进行了子宫内膜活检的前瞻性研究。在人子宫内膜中鉴定出所有四种酶的活性。酸性磷酸酶和N-乙酰-β-D-氨基葡萄糖苷酶的活性相对较高，而α-L-岩藻糖苷酶和α-D-甘露糖苷酶的活性较低。从周期的增殖期到分泌期，这四种酶中任何一种的活性均无显著变化。本研究表明，在正常周期的大部分时间里，这些酶的活性保持恒定。
