Cole S E, Wiltshire T, Rue E E, Morrow D, Hieter P, Brahe C, Fisher E M, Katsanis N, Reeves R H
Department of Physiology, 205, Johns Hopkins University School of Medicine, 725 N. Wolfe St., Baltimore, Maryland 21205, USA.
Mamm Genome. 1999 Mar;10(3):229-34. doi: 10.1007/s003359900978.
Comparative mapping of human and mouse chromosomes can be used to predict locations of homologous loci between the species, provides the substrate to examine the process of chromosomal evolution, and facilitates the continuing development of mouse genetic models for human disorders. A YAC contig of the region of mouse Chromosome (Chr) 10 (MMU10) that demonstrates conserved linkage with the distal portion of human Chr 21 (HSA21) has been constructed. The contig contains all known genes mapped in both species, defines the proximal region of homology between MMU10 and HSA22, and contains the evolutionary junction between HSA21 and HSA22 on MMU10. It consists of 23 YACs and 2 PACs, and covers 3.2 Mb of MMU10. The average marker density for this region is 1 marker/69 kb. Nine of 22 expressed sequences are mapped here for the first time in mouse, and two are newly characterized expressed sequences. The contig also contains 12 simple sequence repeats (SSRs) and 16 YAC and PAC endclone markers. YAC fragmentation analysis was used to create a physical map for the proximal 2.2 Mb of the contig. Cloning of the corresponding region of HSA21 has proven difficult, and the mouse contig includes segments absent from previously described sequence ready maps of HSA21.
人类和小鼠染色体的比较图谱可用于预测物种间同源基因座的位置，为研究染色体进化过程提供基础，并促进针对人类疾病的小鼠遗传模型的持续发展。已构建了小鼠10号染色体（MMU10）区域的酵母人工染色体（YAC）连续克隆系，该区域与人类21号染色体（HSA21）的远端部分显示出保守的连锁关系。该连续克隆系包含两个物种中所有已知定位的基因，确定了MMU10和HSA22之间的同源近端区域，并包含MMU10上HSA21和HSA22之间的进化连接点。它由23个YAC和2个P1人工染色体（PAC）组成，覆盖MMU10的3.2兆碱基对（Mb）。该区域的平均标记密度为1个标记/69千碱基对（kb）。22个表达序列中的9个首次在小鼠中定位于此，还有2个是新鉴定的表达序列。该连续克隆系还包含12个简单序列重复（SSR）以及16个YAC和PAC末端克隆标记。利用YAC片段分析为该连续克隆系近端的2.2 Mb构建了物理图谱。已证实克隆HSA21的相应区域存在困难，并且该小鼠连续克隆系包含了先前描述的HSA21序列就绪图谱中所没有的片段。
