Ugrekhelidze D, Kvesitadze G, Arziani B, Mithaishvili T, Phiriashvili V
Durmishidze Institute of Plant Biochemistry, Academy of Sciences of Georgia, Tbilisi, Digomi, 380059, Georgia, USA.
Ecotoxicol Environ Saf. 1999 Feb;42(2):119-24. doi: 10.1006/eesa.1998.1709.
Transformation of phenol (14C6H5OH) penetrating through the roots of mung bean (Phaseolus aureus) and wheat (Triticum vulgare) sterile seedlings has been studied. Phenol was coupled to low-molecular-weight peptides, producing phenol-peptide conjugates. Hydrolytic cleavage of the conjugates liberated initial labeled phenol and some unlabeled amino acids. Phenol- glutathione and phenol-homoglutathione were not found among the peptide conjugates. It is suggested that the conjugation is carried out via the hydroxyl group of phenol and functional groups of peptides. Conjugation with low-molecular-weight peptides is considered to be the main pathway for phenol detoxication, since about 60% of phenol absorbed by plants conjugates with peptides. In the plants treated with phenol, the amount of low-molecular-weight peptides is increased. The increase in peptide synthesis in plants seems to be induced by the penetration of toxic phenol molecules into the cell. The small amount of phenol molecules assimilated through roots is transformed via aromatic ring cleavage and bibasic carbonic acid formation.
研究了穿透绿豆（Phaseolus aureus）和小麦（Triticum vulgare）无菌幼苗根部的苯酚（¹⁴C₆H₅OH）的转化情况。苯酚与低分子量肽结合，生成苯酚 - 肽缀合物。缀合物的水解裂解释放出初始标记的苯酚和一些未标记的氨基酸。在肽缀合物中未发现苯酚 - 谷胱甘肽和苯酚 - 高半胱氨酸。表明这种结合是通过苯酚的羟基和肽的官能团进行的。与低分子量肽的结合被认为是苯酚解毒的主要途径，因为植物吸收的约60%的苯酚与肽结合。在用苯酚处理的植物中，低分子量肽的量增加。植物中肽合成的增加似乎是由有毒苯酚分子渗透到细胞中诱导的。通过根部吸收的少量苯酚分子通过芳环裂解和二元碳酸形成进行转化。
