Sabelli C A, Popovici A, Kaluza J J, Marcantoni M, Palmieri A, Abramovich A
Rev Asoc Argent Microbiol. 1976 May-Aug;8(2):63-7.
Human teeth were suspended in a basal medium with sucrose 10%; they were incudated for formation of dental plaques "in vitro". The microorganisms used were: Strep. mutans, Lactobacillus salivarius var. salivarius and a mixture of them. The following aspects were stablishad by scanning electron microscopy: 1) The teeth inoculated with Strep. mutans had coccoid forms, lying on an organic film with spheric spaces in some sectors, and irreguar forms in others (Fig. 1). 2) The teeth inoculated with Lactobacillus salivarius had rod forms attached to the adamantine surface (Fig. 2, 3). 3) Teeth incubated with the mixutre had rod forms over a granular film (Fig. 4). 4) The control teath did not have plaque (Fig. 5). This experience shows that the extracellular polisacharide, reticulated or granular (mutano) appeared only in teeth incubated with Strep. mutans alone (Fig. 1) or mixed with Lactobacillus (Fig. 4). On the other hand Lactobacillus appeared attached on the enamel surfaces, perhaps due to cohesive adhesive forces (Fig. 2). Two different interpretation or the formation of dental plaques that take place on the enamel free surfaces of teeth are presented.
将人类牙齿悬浮于含有10%蔗糖的基础培养基中；使其“在体外”孵育以形成牙菌斑。所使用的微生物有：变形链球菌、唾液乳杆菌唾液亚种以及它们的混合物。通过扫描电子显微镜确定了以下几个方面：1）接种变形链球菌的牙齿有球状形态，位于某些区域有球形间隙的有机膜上，其他区域则为不规则形态（图1）。2）接种唾液乳杆菌的牙齿有杆状形态附着于釉质表面（图2、3）。3）与混合物一起孵育的牙齿在颗粒状膜上有杆状形态（图4）。4）对照牙齿没有菌斑（图5）。该实验表明，仅在单独接种变形链球菌（图1）或与乳杆菌混合（图4）孵育的牙齿中出现了网状或颗粒状（变聚糖）细胞外多糖。另一方面，乳杆菌似乎附着在釉质表面，这可能是由于内聚粘附力（图2）。本文提出了关于在牙齿无釉质表面形成牙菌斑的两种不同解释。
