Peterson J E, King M E, Banks W F, Baker J F, Jensen A F, Ross R W, Clemans S, Edelson J
J Pharm Sci. 1978 Oct;67(10):1425-8. doi: 10.1002/jps.2600671026.
A sensitive method is described for the radioimmunoassay of danazol in monkey and human plasma. Antiserum was developed in rabbits, and a second antibody was used to separate bound from free danazol. The radioimmunoassay was specific for danazol, and the limit of detection ranged from 1.4 to 2.8 ng/ml. Exogeneous danazol could be quantitated accurately in both monkey and human plasma. The radioimmunoassay results agreed with values obtained by inverse isotope dilution after intravenous administration of 14C-danazol to monkeys. The assay was used successfully to measure danazol in plasma from human volunteers receiving 200 mg of danazol.
本文描述了一种用于测定猴和人血浆中达那唑的灵敏放射免疫分析方法。用兔制备抗血清，并使用第二抗体将结合态达那唑与游离态达那唑分离。该放射免疫分析方法对达那唑具有特异性，检测限为1.4至2.8 ng/ml。外源性达那唑在猴和人血浆中均可被准确定量。放射免疫分析结果与给猴静脉注射14C-达那唑后通过反向同位素稀释法获得的值一致。该分析方法成功用于测定接受200 mg达那唑的人类志愿者血浆中的达那唑。
