Ishikawa T, Takayama S, Kitagawa T
J Natl Cancer Inst. 1978 Oct;61(4):1101-5.
A system in which the retinal tissues of noninbred Wistar rats were used in combination with autoradiography was developed for measurement of DNA repair synthesis in ganglion cells of the central nervous system. Retinal tissues in short-term organ culture were treated with various carcinogens plus tritiated thymidine ([methyl-3H]dThd) or were irradiated with UV light and then treated with [methyl-3H]dThd. Preliminary study with retinal tissues from rats at various ages revealed no age-associated changes in the levels of unscheduled DNA synthesis in ganglion cells.
开发了一种系统，该系统将非近交系Wistar大鼠的视网膜组织与放射自显影相结合，用于测量中枢神经系统神经节细胞中的DNA修复合成。短期器官培养中的视网膜组织用各种致癌物加氚标记的胸腺嘧啶核苷（[甲基-³H]dThd）处理，或用紫外线照射，然后用[甲基-³H]dThd处理。对不同年龄大鼠视网膜组织的初步研究表明，神经节细胞中DNA合成的水平没有与年龄相关的变化。
