Hamada T, Kako K, Wakamatsu H, Shibata S, Watanabe S, Ishida N
Ishida Group of Clock Gene, National Institute of Bioscience and Human Technology, AIST, MITI, 1-1 Higashi, Tsukuba Science City, 305-8566, Japan.
Brain Res Mol Brain Res. 1999 Mar 5;65(2):211-5. doi: 10.1016/s0169-328x(98)00341-6.
We previously reported the mammalian period repeat mRNA fluctuates during circadian time in the rat suprachiasmatic nucleus (SCN) which is considered to be a clock pacemaker in mammalian brain. Presently we discovered a period repeat sequence (PR) DNA-binding protein in the rat SCN nuclear extract. In the SCN, the binding activity of PR DNA-binding protein to (ACAGGC)3 was most highest during the late day and most lowest during the late night by electro-mobility shift assay (EMSA). In the cortex nuclear extract, the binding of PR DNA-binding protein did not show a significant variation during a day. This is the first report to show the existence of diurnal regulated PR DNA-binding protein in the SCN.
我们之前报道过，哺乳动物周期重复mRNA在大鼠视交叉上核（SCN）的昼夜节律时间内波动，该核被认为是哺乳动物大脑中的一个生物钟起搏器。目前，我们在大鼠SCN核提取物中发现了一种周期重复序列（PR）DNA结合蛋白。通过电泳迁移率变动分析（EMSA），在SCN中，PR DNA结合蛋白与（ACAGGC）3的结合活性在白天晚些时候最高，在深夜最低。在皮质核提取物中，PR DNA结合蛋白的结合在一天中没有显著变化。这是首次报道在SCN中存在昼夜调节的PR DNA结合蛋白。
