Ishida N, Nishimatsu S, Matsui M, Mitsui Y, Nohno T, Shibata N, Noji S
National Institute of Bioscience and Human-Technology, AIST, MITI, Higashi Tsukuba Sience City, Japan.
Neurosci Biobehav Rev. 1994 Winter;18(4):571-7. doi: 10.1016/0149-7634(94)90013-2.
We have recently reported fluctuations in the expression of the period repeat sequence, pp2.5, during light-dark cycles in the suprachiasmatic nucleus (SCN) of rat. Presently, we performed in situ hybridization which shows that the fluctuation of pp2.5 expression continues during constant darkness conditions in the SCN of rat. The light exposure during subjective night but not subjective day triggered its elevated expression in a time-dependent manner which is parallel to that of c-fos expression. In this review, the cloning and characterization of multiple per repeat sequences from mouse genom and rat brain mRNA were summarized. The abundance of a novel per repeat mRNA (designated as RB15) fluctuates during a light-dark cycle in the SCN. These findings suggest that per repeat sequence may have a role for the mammalian circadian rhythms. The evolutionary relationship between the mammarian per repeat sequence and the Drosophila period gene is also discussed.
我们最近报道了大鼠视交叉上核（SCN）中周期重复序列pp2.5在明暗周期中的表达波动。目前，我们进行了原位杂交，结果显示在大鼠SCN的持续黑暗条件下，pp2.5表达的波动仍在继续。主观夜间而非主观白天的光照以时间依赖性方式触发其表达升高，这与c-fos表达平行。在这篇综述中，总结了从小鼠基因组和大鼠脑mRNA中克隆和鉴定多个周期重复序列的情况。一种新型周期重复mRNA（命名为RB15）的丰度在SCN的明暗周期中波动。这些发现表明周期重复序列可能在哺乳动物昼夜节律中起作用。还讨论了哺乳动物周期重复序列与果蝇周期基因之间的进化关系。
