Vaughan P F, Walker J H, Peers C
Institute for Cardiovascular Research, University of Leeds, UK.
Mol Neurobiol. 1998 Oct;18(2):125-55. doi: 10.1007/BF02914269.
The effect of protein kinase C (PKC) on the release of neurotransmitters from a number preparations, including sympathetic nerve endings, brain slices, synaptosomes, and neuronally derived cell lines, is considered. A comparison is drawn between effects of activation of PKC on neurotransmitter release from small synaptic vesicles and large dense-cored vesicles. The enhancement of neurotransmitter release is discussed in relation to the effect of PKC on: 1. Rearrangement of the F-actin-based cytoskeleton, including the possible role of MARCKS in this process, to allow access of large dense-cored vesicles to release sites on the plasma membrane. 2. Phosphorylation of key components in the SNAP/SNARE complex associated with the docking and fusion of vesicles at site of secretion. 3. Ion channel activity, particularly Ca2+ channels.
本文探讨了蛋白激酶C（PKC）对多种制剂中神经递质释放的影响，这些制剂包括交感神经末梢、脑切片、突触体和神经源性细胞系。文中比较了PKC激活对小突触囊泡和大致密核心囊泡神经递质释放的影响。围绕PKC对以下方面的作用，讨论了神经递质释放增强的相关情况：1. 基于F-肌动蛋白的细胞骨架重排，包括MARCKS在此过程中可能发挥的作用，以使大致密核心囊泡能够接近质膜上的释放位点。2. 与囊泡在分泌位点对接和融合相关的SNAP/SNARE复合体关键成分的磷酸化。3. 离子通道活性，特别是钙通道活性。
