Aslani M M, Badami N, Mahmmoodi M, Bouzari S
Microbiology and Molecular Biology Department, Pasteur Institute of Iran, Tehran.
Scand J Infect Dis. 1998;30(5):473-6. doi: 10.1080/00365549850161467.
In a randomly selected population, 2,008 fecal samples were screened for presence of Verotoxin producing Escherichia coli (VTEC) by colony sweep polymyxin-B extraction method. Non-sorbitol fermentation (NSF) phenotype and slide agglutination with O157: H7 antisera were used for screening and detection of this serotype. Ninety-eight (4.9%) fecal samples were found to be VTEC-positives and none of them belonged to the O157: H7 serotype. In rural areas, most individuals carrying VTEC isolates were asymptomatic, whereas in urban areas, a significant association was found between VTEC isolation and diarrhoea (p < 0.01).
在一个随机选取的人群中,采用菌落扫描多粘菌素B提取法对2008份粪便样本进行了产志贺毒素大肠杆菌(VTEC)检测。非山梨醇发酵(NSF)表型和用O157:H7抗血清进行玻片凝集试验用于该血清型的筛查和检测。发现98份(4.9%)粪便样本为VTEC阳性,且均不属于O157:H7血清型。在农村地区,大多数携带VTEC菌株的个体无症状,而在城市地区,发现VTEC分离与腹泻之间存在显著关联(p<0.01)。