Herrmann R, Reiner B
Zentrum für Molekulare Biologie Heidelberg, Mikrobiologie, Universität Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany.
Curr Opin Microbiol. 1998 Oct;1(5):572-9. doi: 10.1016/s1369-5274(98)80091-x.
The rapid progress in sequencing large quantities of DNA will provide an increasing number of complete genome sequences of closely related bacterial species as well as of pairs of isolates from the same species with different features, such as a pathogenic and an apathogenic representative. This opens the way to apply subtractive comparative analysis as a tool to select from the large pool of all bacterial genes a relatively small set of genes that can be correlated with the expression of a certain phenotype. These selected genes can then be the target for further functional analyses.
对大量DNA进行测序的快速进展，将提供越来越多亲缘关系密切的细菌物种的完整基因组序列，以及来自同一物种但具有不同特征（如致病性和非致病性代表菌株）的成对分离株的完整基因组序列。这为应用消减比较分析作为一种工具开辟了道路，以便从所有细菌基因的大集合中选择一组相对较小的、可与特定表型表达相关联的基因。然后，这些选定的基因可成为进一步功能分析的目标。
