Gallie D R
Department of Biochemistry, University of California, Riverside, CA 92521-0129, USA.
Curr Opin Plant Biol. 1998 Apr;1(2):166-72. doi: 10.1016/s1369-5266(98)80020-4.
The repertoire of cis-regulatory elements has increased to a level of sophistication that offers considerable spatial and temporal control over transgene expression. Recent advances made with transgenes have revealed that the control of their expression is also influenced by factors that range from transgene copy number and arrangement to nuclear architecture and chromosomal location. These factors must now be included with the standard considerations of transcriptional and translational enhancers of gene expression during transgene design.
顺式调控元件的种类已增加到一个复杂程度，能够对转基因表达提供相当程度的空间和时间控制。转基因方面的最新进展表明，其表达的控制还受到多种因素的影响，这些因素包括从转基因拷贝数和排列到核结构及染色体位置等。在转基因设计过程中，现在必须将这些因素纳入基因表达的转录和翻译增强子的标准考量之中。
