Sugaya A, Sugiyama T, Yanase S, Terada Y, Toyoda N
Department of Obstetrics and Gynecology, Mie University School of Medicine, Japan.
J Obstet Gynaecol Res. 1999 Feb;25(1):9-14. doi: 10.1111/j.1447-0756.1999.tb01115.x.
To investigate the effects of 17 beta-estradiol and progesterone on the expression of glucose transporter 4 (GLUT4) in the adipose tissue and skeletal-muscle tissue of ovariectomized rats.
Female Sprague-Dawley rats (n = 63) received a daily subcutaneous injection of 10 micrograms, 50 micrograms, or 250 micrograms of 17 beta-estradiol (Group E) or of 1 mg, 5 mg or 25 mg of progesterone (Group P) for 3 days, 7 days, or 10 days (n = 3, at each dose). The expression of GLUT4 mRNA was assessed by performing ribonuclease protection assays.
The levels of GLUT4 mRNA in adipose tissue was significantly reduced by treatment with estradiol, 50 micrograms or 250 micrograms, relative to findings in control rats (p < 0.01). No such reductions were seen regarding the progesterone treatment. The level of GLUT4 mRNA in skeletal-muscle tissue did not change, regardless of treatment.
In ovariectomized rats, higher than physiologic dosages of 17 beta-estradiol can suppress the expression of GLUT4 mRNA in adipose tissue.
研究17β-雌二醇和孕酮对去卵巢大鼠脂肪组织和骨骼肌组织中葡萄糖转运蛋白4（GLUT4）表达的影响。
63只雌性Sprague-Dawley大鼠，每天皮下注射10微克、50微克或250微克的17β-雌二醇（E组）或1毫克、5毫克或25毫克的孕酮（P组），持续3天、7天或10天（每组每种剂量3只大鼠）。通过核糖核酸酶保护试验评估GLUT4 mRNA的表达。
与对照大鼠相比，50微克或250微克雌二醇处理后，脂肪组织中GLUT4 mRNA水平显著降低（p < 0.01）。孕酮处理未观察到这种降低。无论何种处理，骨骼肌组织中GLUT4 mRNA水平均未改变。
在去卵巢大鼠中，高于生理剂量的17β-雌二醇可抑制脂肪组织中GLUT4 mRNA的表达。
