鼻内或皮下联合给予重组霍乱毒素B亚单位，仅能轻微刺激或不能刺激小鼠对破伤风类毒素产生特异性IgE反应。

Intranasal or subcutaneous co-administration of recombinant cholera toxin B subunit stimulates only a slight or no level of the specific IgE response in mice to tetanus toxoid.

作者信息

Isaka M, Yasuda Y, Kozuka S, Taniguchi T, Miura Y, Matano K, Goto N, Tochikubo K

机构信息

Department of Microbiology, Nagoya City University Medical School, Nagoya, Japan.

出版信息

Vaccine. 1999 Feb 26;17(7-8):944-8. doi: 10.1016/s0264-410x(98)00280-1.

DOI:10.1016/s0264-410x(98)00280-1

Abstract

Whether recombinant cholera toxin B subunit (rCTB) co-administered intranasally or subcutaneously with aluminium-non-adsorbed tetanus toxoid (nTT) can induce the production of tetanus toxoid (TT)-specific IgE antibodies in mice was investigated compared with aluminium-adsorbed tetanus toxoid (aTT) administered intranasally or subcutaneously. Mice immunized intranasally or subcutaneously with nTT together with rCTB showed a high level of TT-specific serum IgG antibody response and no or a slight level of TT-specific serum IgE antibody response. On the other hand, in mice vaccinated intranasally or subcutaneously with aTT alone, higher levels of TT-specific IgG and IgE antibodies were induced in comparison with intranasal or subcutaneous inoculation of nTT together with rCTB. These results suggest that intranasal or subcutaneous co-administration of rCTB with nTT is better than intranasal or subcutaneous administration of aTT to avoid IgE-mediated allergic reactions.

摘要

将重组霍乱毒素B亚基（rCTB）与未吸附铝的破伤风类毒素（nTT）经鼻内或皮下联合给药，与经鼻内或皮下给予吸附铝的破伤风类毒素（aTT）相比，研究其是否能在小鼠体内诱导产生破伤风类毒素（TT）特异性IgE抗体。经鼻内或皮下用nTT与rCTB联合免疫的小鼠表现出高水平的TT特异性血清IgG抗体反应，且TT特异性血清IgE抗体反应无或水平轻微。另一方面，与单独经鼻内或皮下接种nTT与rCTB相比，单独经鼻内或皮下接种aTT的小鼠诱导产生了更高水平的TT特异性IgG和IgE抗体。这些结果表明，rCTB与nTT经鼻内或皮下联合给药比aTT经鼻内或皮下给药更有利于避免IgE介导的过敏反应。