Kolmac C, Mitrofanis J
Institute für Biomedical Research, Department of Anatomy and Histology, University of Sydney, Australia.
Anat Embryol (Berl). 1999 Mar;199(3):265-80. doi: 10.1007/s004290050227.
To gain insight into the cellular organisation of the zona incerta, we have examined the chemoarchitectonic properties of this "uncertain zone". The brains of Sprague-Dawley rats and common cats were processed for immunocytochemistry or NADPH-diaphorase histochemistry using standard methods. For the immunocytochemistry, antibodies to y-aminobutyric acid (GABA), glutamic acid decarboxylase (GAD), parvalbumin, calbindin, tyrosine hydroxylase, somatostatin, serotonin and glutamate were used. Two general patterns of distribution in the zona incerta were seen. First, labelled cells were restricted largely to one of the cytoarchitectonically defined sectors of the zona incerta. For instance, GABA, GAD and parvalbumin-immunoreactive cells were found principally within the ventral sector, NADPH-diaphorase and glutamate-immunoreactive cells within the dorsal sector and tyrosine hydroxylase- and somatostatin-immunoreactive cells within the rostral sector. Second, labelled cells were scattered somewhat across all incertal sectors, with no clear region of concentration. This pattern included the calbindin- and serotonin-immunoreactive cell groups. These results indicate that the zona incerta is made up of many neurochemically distinct cell groups, some of which respect the well-defined cytoarchitectonic boundaries of the nucleus, whilst others do not. This rich neurochemical diversity in the zona incerta suggests that this nucleus may have differential effects on the different structures that it projects to.
为深入了解未定带的细胞组织，我们研究了这个“不确定区域”的化学构筑特性。采用标准方法对Sprague-Dawley大鼠和普通猫的大脑进行免疫细胞化学或NADPH-黄递酶组织化学处理。对于免疫细胞化学，使用了针对γ-氨基丁酸（GABA）、谷氨酸脱羧酶（GAD）、小白蛋白、钙结合蛋白、酪氨酸羟化酶、生长抑素、5-羟色胺和谷氨酸的抗体。在未定带观察到两种一般的分布模式。首先，标记细胞主要局限于未定带细胞构筑学定义的一个区域。例如，GABA、GAD和小白蛋白免疫反应阳性细胞主要见于腹侧区域，NADPH-黄递酶和谷氨酸免疫反应阳性细胞见于背侧区域，酪氨酸羟化酶和生长抑素免疫反应阳性细胞见于嘴侧区域。其次，标记细胞在所有未定带区域均有一定程度的散在分布，没有明显的集中区域。这种模式包括钙结合蛋白和5-羟色胺免疫反应阳性细胞群。这些结果表明，未定带由许多神经化学性质不同的细胞群组成，其中一些细胞群遵循该核明确的细胞构筑边界，而另一些则不然。未定带丰富的神经化学多样性表明，该核可能对其投射到的不同结构有不同的影响。
