Kontula P, Suihko M L, Von Wright A, Mattila-Sandholm T
VTT Biotechnology and Food Research, Finland.
J Dairy Sci. 1999 Feb;82(2):249-56. doi: 10.3168/jds.S0022-0302(99)75230-6.
Nine strains of lactic acid bacteria were studied for growth and fermentation end products on lactulose, lactitol, and lactobionic acid. In addition, human fecal and biopsy isolates were screened for new potential by probiotic strains utilizing lactose derivatives, and one new isolate of Lactobacillus rhamnosus was enriched. The utilization of lactose derivatives and the effect on the fermentation end products were dependent on strain. Typical mixed-acid fermentations were observed with Lb. rhamnosus and Lactococcus lactis. Microbiota enriched from fecal and biopsy samples using modified MRS medium consisted mainly of enterococci and streptococci. The adhesion of tested strains to Caco-2 cells was not dependent on carbon source. The new Lb. rhamnosus strain VTT E-97800 has potential for further probiotic studies.
研究了9株乳酸菌在乳果糖、乳糖醇和乳糖酸上的生长及发酵终产物。此外，对人粪便和活检分离株进行筛选，以寻找利用乳糖衍生物的新的潜在益生菌菌株，并富集了一株新的鼠李糖乳杆菌分离株。乳糖衍生物的利用情况以及对发酵终产物的影响因菌株而异。用鼠李糖乳杆菌和乳酸乳球菌观察到典型的混合酸发酵。使用改良MRS培养基从粪便和活检样本中富集的微生物群主要由肠球菌和链球菌组成。受试菌株对Caco-2细胞的黏附不依赖于碳源。新的鼠李糖乳杆菌菌株VTT E-97800有进一步进行益生菌研究的潜力。
