Yang W Z, Beauchemin K A, Rode L M
Livestock Sciences Section, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada.
J Dairy Sci. 1999 Feb;82(2):391-403. doi: 10.3168/jds.S0022-0302(99)75245-8.
A study was conducted using lactating Holstein cows with ruminal and duodenal cannulas in a 4 x 4 Latin square design to investigate fibrolytic enzyme supplementation on site and extent of nutrient digestion. The four diets consisted of 45% concentrate, 10% barley silage, and 45% cubed alfalfa hay (dry matter basis) and differed in enzyme supplementation: 1) control cubes, 2) cubes treated with 1 g of enzyme mixture/kg of cubes, 3) cubes treated with 2 g of enzyme mixture/kg of cubes, and 4) both concentrate and cubes treated with 1 g of enzyme mixture/kg of dry matter. The enzyme supplement contained primarily cellulase and xylanase activities. Digestion of organic matter and neutral detergent fiber in the total tract was higher for cows fed the high dosage of enzyme than for cows fed the control cubes. Ruminal digestibility of crude protein was higher, but that of organic matter and neutral detergent fiber was only numerically higher, for cows fed the high dosage of enzyme compared with that of cows fed the control cubes. Higher ruminal digestibility associated with the high dosage of enzyme resulted in more microbial protein synthesis. Milk production increased for cows fed the high dosage of enzyme compared with cows fed the control cubes and effects of the addition of enzyme on milk composition were minimal. The results demonstrated the benefits of using a fibrolytic enzyme additive to enhance feed digestion and milk production by dairy cows. The response to enzyme supplementation was affected more by amount of enzyme than by whether the enzyme was added to forage or concentrate.
采用4×4拉丁方设计,对装有瘤胃和十二指肠瘘管的泌乳荷斯坦奶牛进行了一项研究,以调查添加纤维分解酶对养分消化部位和程度的影响。四种日粮均由45%的精料、10%的大麦青贮和45%的苜蓿草块(干物质基础)组成,酶添加量不同:1)对照草块;2)每千克草块用1克酶混合物处理的草块;3)每千克草块用2克酶混合物处理的草块;4)精料和草块均按每千克干物质用1克酶混合物处理。酶添加剂主要含有纤维素酶和木聚糖酶活性。高剂量酶组奶牛的全消化道有机物和中性洗涤纤维消化率高于对照草块组奶牛。与对照草块组奶牛相比,高剂量酶组奶牛的瘤胃粗蛋白消化率更高,但有机物和中性洗涤纤维的瘤胃消化率仅在数值上更高。高剂量酶导致的较高瘤胃消化率使得微生物蛋白合成增加。与对照草块组奶牛相比,高剂量酶组奶牛的产奶量增加,且添加酶对牛奶成分的影响最小。结果表明,使用纤维分解酶添加剂可提高奶牛的饲料消化率和产奶量。酶添加量对酶添加反应的影响大于酶添加于草料还是精料。