须癣毛癣菌菌丝期及关节孢子期抗原的检测

Detection of antigens in mycelial and in arthroconidial phases of Trichophyton mentagrophytes.

作者信息

Kopecek P, Weigl E, Raska M

机构信息

Department of Biology, Medical Faculty, Palacký University, Olomouc, Czech Republic.

出版信息

Folia Microbiol (Praha). 1998;43(6):702-6. doi: 10.1007/BF02816394.

DOI:10.1007/BF02816394

PMID:10069016

Abstract

Protein pattern changes were investigated in the filamentous fungus Trichophyton mentagrophytes during the morphological transition induced by increased temperature and higher CO2 partial pressure in cultivation atmosphere. The differences between the mycelial and the arthroconidial phase were characterized by SDS-PAGE and by immunodetection with mouse polyclonal antibodies. The components found by Western blotting in mycelia were 88, 86, 32, 29, 19.5, 18.5 kDa, in arthroconidia 108, 92, 88, 66, 56, 41, 39, 19.5 kDa. The results suggest the participation of some heat shock associated proteins of T. mentagrophytes in host immune response against mycotic infection.

摘要

在培养环境中，通过提高温度和增加二氧化碳分压诱导丝状真菌须癣毛癣菌发生形态转变，在此过程中对其蛋白质模式变化进行了研究。通过SDS-PAGE和用小鼠多克隆抗体进行免疫检测，对菌丝体阶段和关节孢子阶段之间的差异进行了表征。通过蛋白质免疫印迹法在菌丝体中发现的组分有88、86、32、29、19.5、18.5 kDa，在关节孢子中发现的组分有108、92、88、66、56、41、39、19.5 kDa。结果表明，须癣毛癣菌的一些热休克相关蛋白参与了宿主针对真菌感染的免疫反应。