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一种用于脂质指纹图谱的一步荧光检测方法;铕(噻吩甲酰三氟丙酮)3·2三正辛基氧化膦

A one-step fluorescent detection method for lipid fingerprints; Eu(TTA)3.2TOPO.

作者信息

Wilkinson D

机构信息

Forensic Identification Research and Review Section, Royal Canadian Mounted Police, Ottawa, Ontario, Canada.

出版信息

Forensic Sci Int. 1999 Jan 4;99(1):5-23. doi: 10.1016/s0379-0738(98)00176-5.

Abstract

This paper describes the development of a one-step fluorescent lipid reagent that involves europium chelates whose generic formula can be represented as europium(organic ligand)3.2(synergic reagent). An optimized formulation is presented and a comparison with physical developer was conducted which showed the new technique to be very good when fresh fingerprints were recovered but inferior to physical developer on aged prints (over a week). In addition, the compatibility of the technique with sequential methods such as ninhydrin and DFO is reported. The experimentation, results and chemical formulation of this study were presented to delegates of the International Symposium on Fingerprint Detection and Identification held in Israel in June 1995. Prior to that a patent application had been made.

摘要

本文描述了一种一步法荧光脂质试剂的研发情况,该试剂涉及铕螯合物,其通式可表示为铕(有机配体)₃·2(协同试剂)。文中给出了优化配方,并与物理显影剂进行了比较,结果表明,对于新鲜指纹,新技术效果很好,但对于陈旧指纹(一周以上),其效果不如物理显影剂。此外,还报告了该技术与茚三酮和DFO等顺序方法的兼容性。本研究的实验、结果和化学配方已在1995年6月于以色列举行的国际指纹检测与识别研讨会上向与会代表展示。在此之前已提交了专利申请。

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