Chávez E, Zazueta C, García N
Departamento de Bioquímica, Instituto Nacional de Cardiologia, Ignacio Chávez, Mexico DF, Mexico.
FEBS Lett. 1999 Feb 19;445(1):189-91. doi: 10.1016/s0014-5793(99)00128-3.
Addition of a low concentration of carboxyatractyloside (0.075 microM) renders mitochondria susceptible to the opening of the non-specific pore by 5 microM oleate, in a cyclosporin A-sensitive fashion. Matrix Ca2+ efflux as well as collapse of the transmembrane potential reveal permeability transition. The effect of oleate is reached after the titration, by carboxyatractyloside, of 38 pmol of adenine nucleotide translocase per mg mitochondrial protein. We propose that permeability transition may result from an additive action of carboxyatractyloside plus oleate on the ADP/ATP carrier.
添加低浓度的羧基苍术苷（0.075微摩尔）可使线粒体在环孢菌素A敏感的情况下，易被5微摩尔油酸诱导打开非特异性孔道。基质Ca2+外流以及跨膜电位的崩溃揭示了通透性转变。在以羧基苍术苷滴定每毫克线粒体蛋白38皮摩尔腺嘌呤核苷酸转位酶后，可观察到油酸的这种作用。我们提出，通透性转变可能是羧基苍术苷和油酸对ADP/ATP载体的累加作用所致。
