Harper G, Osuji J O, Heslop-Harrison J S, Hull R
John Innes Centre, Norwich Research Park, Colney, NR4 7UH, United Kingdom.
Virology. 1999 Mar 15;255(2):207-13. doi: 10.1006/viro.1998.9581.
Breeding and tissue culture of certain cultivars of bananas (Musa) have led to high levels of banana streak badnavirus (BSV) infection in progeny from symptomless parents. BSV DNA hybridized to genomic DNA of one such parent, Obino l'Ewai, suggesting integration of viral sequences. Sequencing of clones of Obino l'Ewai genomic DNA revealed an interface between BSV and Musa sequences and a complex BSV integrant. In situ hybridization revealed two different BSV sequence locations in Obino l'Ewai chromosomes and a complex arrangement of BSV and Musa sequences was shown by probing stretched DNA fibers. This is the first report of integrated sequences that possibly lead to a plant pararetrovirus episomal infection by a mechanism differing markedly from animal retroviral systems.
某些香蕉（芭蕉属）品种的育种和组织培养导致了无症状亲本后代中香蕉条纹巴德那病毒（BSV）的高感染率。BSV DNA与其中一个这样的亲本奥比诺·勒瓦伊的基因组DNA杂交，表明病毒序列发生了整合。对奥比诺·勒瓦伊基因组DNA克隆进行测序，揭示了BSV与芭蕉属序列之间的一个界面以及一个复杂的BSV整合体。原位杂交揭示了奥比诺·勒瓦伊染色体中两个不同的BSV序列位置，通过探测拉伸的DNA纤维显示出BSV和芭蕉属序列的复杂排列。这是关于整合序列的首次报道，这些序列可能通过一种与动物逆转录病毒系统明显不同的机制导致植物副逆转录病毒的附加体感染。
