Combrisson J, Monrozier L J
Laboratoire d'Ecologie Microbienne du Sol, UMR CNRS 5557, Université Claude Bernard Lyon I, Villeurbanne, France.
Chemosphere. 1999 Mar;38(6):1305-11. doi: 10.1016/s0045-6535(98)00531-1.
We examined the mineralization of pentachlorophenol (PCP) in sterile and non-sterile soil with or without added bacteria (Mycobacterium chlorophenolicum PCP-1). The soil used had no history of PCP contamination. Microcosms (30 g dry weight of soil) were incubated with labelled PCP (6.76% 13C, a non-radioactive stable isotope, 22 mg kg-1 dry weight) for 60 days. M. chlorophenolicum PCP-1 (7.8 x 10(6) cells g-1 dry weight) was added to some samples. 50% of the PCP was mineralized in non-sterile soil with or without the exogenous bacteria. Only 5% of the PCP was mineralized in sterile soil with or without bacteria. These data suggest that the PCP was not accessible to M. chlorophenolicum and that the indigenous soil microflora can mineralize PCP.
我们研究了在添加或不添加细菌（氯酚分枝杆菌PCP-1）的无菌和非无菌土壤中五氯苯酚（PCP）的矿化情况。所使用的土壤没有PCP污染历史。将微型生态系统（30克干重土壤）与标记的PCP（6.76% 13C，一种非放射性稳定同位素，22毫克/千克干重）一起培养60天。向一些样品中添加了氯酚分枝杆菌PCP-1（7.8×10(6)个细胞/克干重）。在添加或不添加外源细菌的非无菌土壤中，50%的PCP被矿化。在添加或不添加细菌的无菌土壤中，只有5%的PCP被矿化。这些数据表明，氯酚分枝杆菌无法接触到PCP，并且土壤中的土著微生物群落能够使PCP矿化。
