采用毛细管电泳法测定一种眼部护理药物中的 tromethamine（三羟甲基氨基甲烷）含量。
Determination of tromethamine in an eye-care pharmaceutical by capillary electrophoresis.
作者信息
McArdle F A, Meehan C J
机构信息
Department of Applied Science, Institute of Technology, Sligo, Ireland.
出版信息
Analyst. 1998 Aug;123(8):1757-60. doi: 10.1039/a802417k.
Tromethamine [tris(hydroxymethyl)aminomethane] is used as an emulsifying agent, alkaliser and buffering agent in pharmaceutical preparations such as eye-care solutions. A new method for the determination of this compound in an eye-care preparation was developed using capillary zone electrophoresis with indirect UV detection. The method displayed linearity between 2.5 and 25 mg l-1 with a correlation coefficient of 0.9992. The limit of detection was 2.5 mg l-1. Precision and accuracy were determined as relative standard deviation and % deviation, and were found to be 0.42% and 1.62%, respectively. Recovery studies of tromethamine in the pharmaceutical preparation gave a 102.98% recovery.
三羟甲基氨基甲烷[三（羟甲基）氨基甲烷]在眼护理溶液等药物制剂中用作乳化剂、碱化剂和缓冲剂。采用毛细管区带电泳间接紫外检测法建立了一种测定眼护理制剂中该化合物的新方法。该方法在2.5至25 mg l-1之间呈线性，相关系数为0.9992。检测限为2.5 mg l-1。精密度和准确度分别以相对标准偏差和偏差百分比来确定，结果分别为0.42%和1.62%。对该药物制剂中三羟甲基氨基甲烷的回收率研究得出回收率为102.98%。
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