Tobin P C, Pitts C W
Department of Entomology, Pennsylvania State University, University Park 16802-3508, USA.
J Med Entomol. 1999 Jan;36(1):121-3. doi: 10.1093/jmedent/36.1.121.
We examined the efficacy of a sucrose-based flotation method for extracting 3 prevalent insect species from poultry house manure: 3rd-instar Musca domestica L. (Diptera: Muscidae), Carcinops pumilio (Erichson) (Coleoptera: Histeridae) adults, and Alphitobius diaperinus (Panzer) (Coleoptera: Tenebrionidae) adults. A 0.6 M sucrose solution in distilled water altered the specific gravity to 1.2 and caused the 3 species to float to the water surface for easy separation. An analysis of variance (ANOVA) showed significant effects of the solution volume to sample mass ratio on recovery rates (F = 35.96, P < 0.0001), but no differences in species-specific recovery rates (F = 0.97, P = 0.3824). Among samples agitated in a ratio of at least 3.2 ml solution per gram of sample mass, we observed neither significant ratio effects (F = 1.37, P = 0.2442) nor significant differences in species-specific recovery rates (F = 2.05, P = 0.1353); overall, we recovered 97.49 +/- 0.20% of all individuals of the 3 species. Our analyses demonstrated that sucrose-based flotation was a statistically valid method of collecting these species from poultry manure samples.
我们研究了一种基于蔗糖的浮选方法从禽舍粪便中提取3种常见昆虫的效果：家蝇（双翅目：蝇科）三龄幼虫、矮小隐翅虫（鞘翅目：隐翅虫科）成虫以及赤拟谷盗（鞘翅目：拟步甲科）成虫。蒸馏水中0.6 M的蔗糖溶液将比重改变至1.2，使这3个物种漂浮至水面以便于分离。方差分析（ANOVA）表明溶液体积与样品质量比显著影响回收率（F = 35.96，P < 0.0001），但物种特异性回收率无差异（F = 0.97，P = 0.3824）。在以每克样品质量至少3.2毫升溶液的比例搅拌的样品中，我们既未观察到显著的比例效应（F = 1.37，P = 0.2442），也未观察到物种特异性回收率的显著差异（F = 2.05，P = 0.1353）；总体而言，我们回收了这3个物种所有个体的97.49 +/- 0.20%。我们的分析表明，基于蔗糖的浮选是从家禽粪便样品中收集这些物种的一种统计学上有效的方法。
