Fukushima C, Shimoda T, Matsuse H, Matsuo N, Takao A, Obase Y, Asai S, Ayabe H, Kohno S
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Ann Allergy Asthma Immunol. 1999 Feb;82(2):179-84. doi: 10.1016/S1081-1206(10)62594-X.
The airway of asthmatic patients is hyperresponsive to various stimuli in vivo. There are, however, only a few reports that compared the in vivo responsiveness of asthmatic patients and non-asthmatic subjects to those of lung parenchyma in vitro.
To compare the contractile response, release of various chemical mediators, and responsiveness to drugs in samples of lung parenchyma excised from asthma patients with those of non-asthmatic subjects.
Human lung parenchymal strips were subjected to passive sensitization with sera of 5+ RAST titer to mites. The strip was suspended in a magnus bath containing a buffer solution. Parenchymal contraction was induced by PGF2 alpha. After washing, the baseline concentrations of thromboxane B2 (TXB2), leukotriene (LT), and histamine were measured in each bath and then contraction was induced by the addition of a mite antigen. The concentrations of TXB2, LT, and histamine were measured after contraction. The inhibitory effects of TXA2 synthetase inhibitor (DP-1904) and TXA2 receptor antagonist (AA-2414) were also evaluated in both tissue samples.
There were no significant differences between lung parenchymal tissues of asthmatic and non-asthmatic patients with regard to PGF2 alpha-induced contraction, antigen-induced contraction, release of chemical mediators, and the response to drugs.
Unlike the response in vivo, there are no differences in the response to stimuli in vitro between lung parenchymal tissues of asthmatic and non-asthmatic patients.
哮喘患者的气道在体内对各种刺激具有高反应性。然而，仅有少数报告比较了哮喘患者和非哮喘受试者在体内的反应性与肺实质在体外的反应性。
比较从哮喘患者切除的肺实质样本与非哮喘受试者的肺实质样本在收缩反应、各种化学介质释放及对药物的反应性方面的差异。
用人抗螨5级以上RAST效价血清对人肺实质条进行被动致敏。将条带悬挂于含缓冲溶液的马格努斯浴槽中。用前列腺素F2α诱导实质收缩。冲洗后，测量各浴槽中血栓素B2（TXB2）、白三烯（LT）和组胺的基线浓度，然后加入螨抗原诱导收缩。收缩后测量TXB2、LT和组胺的浓度。还评估了血栓素A2合成酶抑制剂（DP - 1904）和血栓素A2受体拮抗剂（AA - 2414）对两种组织样本的抑制作用。
哮喘患者和非哮喘患者的肺实质组织在前列腺素F2α诱导的收缩、抗原诱导的收缩、化学介质释放及对药物的反应方面无显著差异。
与体内反应不同，哮喘患者和非哮喘患者的肺实质组织在体外对刺激的反应无差异。
