Maćkowiak P, Nogowski L, Nowak K W
Department of Animal Physiology and Biochemistry, Poznań University of Agriculture, Poland.
J Recept Signal Transduct Res. 1999 Jan-Jul;19(1-4):283-92. doi: 10.3109/10799899909036651.
The experiments were carried out on ovariectomized Wistar rats. Their livers were perfused with basic perfusion medium (BPM) or BPM supplemented with isoflavone genistein, insulin or combination of the two factors. The obtained results support the hypothesis that genistein influences the kinetics of insulin binding to cell membranes changing the number of insulin receptors and dissociation constant (Kd). BPM supplementation with genistein decreased number of high affinity insulin receptors (HAIR) both in livers treated and untreated with insulin. The amount of HAIR diminished significantly from 610 +/- 77 x 10(-15) (no genistein) to 238 +/- 72 x 10(-15) mol/mg of membrane protein (supplement of genistein). Similarly, genistein reduced slightly the amount of HAIR even when added together with insulin (372 +/- 59 x 10(-15) mol/mg) in comparison to rats perfused with medium containing insulin but not the isoflavone (421 +/- 46 x 10(-15) mol/mg). Simultaneously, genistein decreased significantly Kd for HAIR (perfusion with BPM--1.44 +/- 0.18 x 10(-9) mol/l; perfusion with BMP + genistein--0.83 +/- 0.20 x 10(-9) mol/l). Such effects of genistein during liver perfision did not take place when the liver membranes were in vitro incubated with this xenobiotic.
实验在去卵巢的Wistar大鼠身上进行。用基础灌注培养基（BPM）或添加了异黄酮染料木黄酮、胰岛素或这两种因子组合的BPM对它们的肝脏进行灌注。所得结果支持这样的假设，即染料木黄酮通过改变胰岛素受体数量和解离常数（Kd）来影响胰岛素与细胞膜结合的动力学。用染料木黄酮补充BPM会使无论是否用胰岛素处理的肝脏中高亲和力胰岛素受体（HAIR）的数量减少。HAIR的量从610±77×10⁻¹⁵（无染料木黄酮）显著减少至238±72×10⁻¹⁵摩尔/毫克膜蛋白（染料木黄酮补充组）。同样，与灌注含胰岛素但不含异黄酮的培养基的大鼠相比，即使将染料木黄酮与胰岛素一起添加，其也会使HAIR的量略有减少（372±59×10⁻¹⁵摩尔/毫克）。同时，染料木黄酮使HAIR的Kd显著降低（用BPM灌注时为1.44±0.18×10⁻⁹摩尔/升；用BMP +染料木黄酮灌注时为0.83±0.20×10⁻⁹摩尔/升）。当肝细胞膜与这种异生物在体外孵育时，染料木黄酮在肝脏灌注过程中并未产生这样的效果。
