来自桑树的莫兰20K的纯化与表征

Purification and characterization of Moran 20K from Morus alba.

作者信息

Kim E S, Park S J, Lee E J, Kim B K, Huh H, Lee B J

机构信息

Department of Pharmacy, Seoul National University, Korea.

出版信息

Arch Pharm Res. 1999 Feb;22(1):9-12. doi: 10.1007/BF02976428.

DOI:10.1007/BF02976428

PMID:10071952

Abstract

A new glycoprotein was purified from the aqueous methanolic extract of the root bark of Morus alba which has been used as a component of antidiabetic remedy in Oriental Medicine. SDS-PAGE result shows that the molecular weight of the glycoprotein was approximately 20 kDa. This new glycoprotein was named as Moran 20K. The protein lowered blood glucose level in streptozotocin-induced hyperglycemic mice model and it also increased the glucose transport in cultured epididymis fat cells. The amino acid composition of the protein was analyzed, and the protein contained above 20% serine and cysteine such as insulin. The actual molecular weight of the protein was determined as 21,858 Da by MALDI-TOF mass spectroscopy.

摘要

从桑白皮的甲醇水提取物中纯化出一种新的糖蛋白，在东方医学中，该提取物一直被用作抗糖尿病药物的一种成分。SDS-PAGE结果表明，该糖蛋白的分子量约为20 kDa。这种新的糖蛋白被命名为Moran 20K。该蛋白可降低链脲佐菌素诱导的高血糖小鼠模型的血糖水平，还能增加培养的附睾脂肪细胞中的葡萄糖转运。对该蛋白的氨基酸组成进行了分析，发现该蛋白含有超过20%的丝氨酸和半胱氨酸，与胰岛素类似。通过基质辅助激光解吸电离飞行时间质谱法测定该蛋白的实际分子量为21,858 Da。