Bergdahl A, Valdemarsson S, Nilsson T, Sun X Y, Hedner T, Edvinsson L
Department of Internal Medicine, Lund University, Sweden.
Acta Physiol Scand. 1999 Jan;165(1):15-23. doi: 10.1046/j.1365-201x.1999.00456.x.
It was examined to what extent congestive heart failure (CHF) in rats, induced by ligation of the left coronary artery, affects the vascular responses to the vasodilatory substances acetylcholine (ACh), calcitonin gene-related peptide (CGRP), and substance P (SP). After induction of CHF status, the basilar, mesenteric and renal arteries and the iliac vein were studied in vitro. Dilatory responses were determined in relation to pre-contraction by the thromboxane mimetic U46619. Sham-operated animals (Sham) served as controls. U46619 induced stronger contraction in CHF basilar and renal arteries compared with the corresponding segments in Sham. ACh induced concentration-dependent dilations in all vessels examined with no difference of maximum relaxation or potency between CHF and Sham. SP induced weak dilations in all arteries examined while the response was markedly attenuated in CHF iliac veins compared with Sham (Emax% 12.2 +/- 3.4 vs. 32.3 +/- 4.8, P = 0.01). The CGRP induced dilation in the CHF basilar artery was weaker (Emax% 18.6 +/- 6.5 vs. 66.9 +/- 5.0, P < 0.001) and less potent (pEC50: 8.2 +/- 0.2 vs. 9.0 +/- 0.2, P = 0.01) compared with Sham. Further, CGRP was less potent in the renal artery of CHF rats compared with Sham (pEC50: 8.1 +/- 0.2 vs. 9.5 +/- 0.3, P < 0.01). In the CHF iliac vein, CGRP was more potent compared with Sham (pEC50: 9.7 +/- 0.4 vs. 8.3 +/- 0.4, P < 0.05). It can be concluded CHF is accompanied by alterations in the vascular response to the dilatory substances studied. The changes differ between vascular beds and between the different substances.
研究了通过结扎左冠状动脉诱导大鼠充血性心力衰竭（CHF）对血管对血管舒张物质乙酰胆碱（ACh）、降钙素基因相关肽（CGRP）和P物质（SP）反应的影响程度。在诱导CHF状态后，对基底动脉、肠系膜动脉、肾动脉和髂静脉进行体外研究。通过血栓素类似物U46619预收缩来测定舒张反应。假手术动物（Sham）作为对照。与Sham组相应节段相比，U46619在CHF基底动脉和肾动脉中诱导更强的收缩。ACh在所有检测血管中诱导浓度依赖性舒张，CHF组和Sham组之间最大舒张或效能无差异。SP在所有检测动脉中诱导微弱舒张，而与Sham组相比，CHF髂静脉中的反应明显减弱（最大效应百分比：12.2±3.4对32.3±4.8，P = 0.01）。与Sham组相比，CGRP在CHF基底动脉中诱导的舒张较弱（最大效应百分比：18.6±6.5对66.9±5.0，P < 0.001）且效能较低（pEC50：8.2±0.2对9.0±0.2，P = 0.01）。此外，与Sham组相比，CGRP在CHF大鼠肾动脉中的效能较低（pEC50：8.1±0.2对9.5±0.3，P < 0.01）。在CHF髂静脉中，与Sham组相比，CGRP效能更高（pEC50：9.7±0.4对8.3±0.4，P < 0.05）。可以得出结论，CHF伴随着对所研究舒张物质的血管反应改变。这些变化在不同血管床和不同物质之间存在差异。
