[缺氧和牛磺酸对培养的牛肺动脉内皮细胞血管收缩肽的影响]
[Effects of hypoxia and taurine on vasoconstriction peptides from cultured bovine pulmonary arterial endothelial cells].
作者信息
Yu Z, Li J, Sun B
机构信息
Department of Respiratory Disease, Beijing Military General Hospital.
出版信息
Zhonghua Jie He He Hu Xi Za Zhi. 1997 Feb;20(1):25-9.
OBJECTIVE
To study roles of endothein-1 (ET-1), angiotensin-II (AT-II) and endogenous digitalis-like factor (EDF) from cultured bovine pulmonary arterial endothelial cells (PAEC) in the pathogenesis of hypoxic pulmonary hypertension, and to evaluate whether Taurine is capable of protecting tissue cells from injury.
METHODS
Culture of bovine pulmonary arterial endothelial cells and dot blot hybridization.
RESULTS
PAEC cultured under hypoxia resulted in an increase in ET-1 mRNA expression. PAEC cultured under hypoxia induced increase in ET-1, AT-II and EDF release. By adding taurine to the culture medium, ET-1 mRNA express was inhibited. ET-1, AT-II and EDF release were inhibited.
CONCLUSIONS
Expression of ET-1 mRNA and release of ET-1, AT-II and EDF from PAEC increased under hypoxia. Taurine can inhibit the hypoxia-induced expression of ET-1 mRNA of PAEC and reduce the release of ET-1, AT-II and EDF from PAEC.
目的
研究培养的牛肺动脉内皮细胞（PAEC）中内皮素-1（ET-1）、血管紧张素-II（AT-II）和内源性类洋地黄因子（EDF）在低氧性肺动脉高压发病机制中的作用，并评估牛磺酸是否能够保护组织细胞免受损伤。
方法
牛肺动脉内皮细胞培养及斑点杂交。
结果
低氧培养的PAEC导致ET-1 mRNA表达增加。低氧培养的PAEC诱导ET-1、AT-II和EDF释放增加。向培养基中添加牛磺酸可抑制ET-1 mRNA表达。ET-1、AT-II和EDF释放受到抑制。
结论
低氧条件下PAEC中ET-1 mRNA的表达以及ET-1、AT-II和EDF的释放增加。牛磺酸可抑制PAEC低氧诱导的ET-1 mRNA表达，并减少PAEC中ET-1、AT-II和EDF的释放。
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