[Effects of hypoxia and taurine on vasoconstriction peptides from cultured bovine pulmonary arterial endothelial cells].

OBJECTIVE

To study roles of endothein-1 (ET-1), angiotensin-II (AT-II) and endogenous digitalis-like factor (EDF) from cultured bovine pulmonary arterial endothelial cells (PAEC) in the pathogenesis of hypoxic pulmonary hypertension, and to evaluate whether Taurine is capable of protecting tissue cells from injury.

METHODS

Culture of bovine pulmonary arterial endothelial cells and dot blot hybridization.

RESULTS

PAEC cultured under hypoxia resulted in an increase in ET-1 mRNA expression. PAEC cultured under hypoxia induced increase in ET-1, AT-II and EDF release. By adding taurine to the culture medium, ET-1 mRNA express was inhibited. ET-1, AT-II and EDF release were inhibited.

CONCLUSIONS

Expression of ET-1 mRNA and release of ET-1, AT-II and EDF from PAEC increased under hypoxia. Taurine can inhibit the hypoxia-induced expression of ET-1 mRNA of PAEC and reduce the release of ET-1, AT-II and EDF from PAEC.