Wang J, Wang Z, Chen M
First Affiliated Hospital, West China University of Medical Sciences, Chengdu.
Zhonghua Jie He He Hu Xi Za Zhi. 1997 Jun;20(3):141-4.
To observe the relationship among Lung cancer cell apoptosis, proliferation and related gene expression and its significance as an index of prognosis.
In situ end labelling (ISEL) technique and DNA gel electrophoresis were used to detect the apoptotic cell, immunohistochemistry was used to detect the expression of bcl-2, P53 and ki-67 in 57 cases of Lung cancer.
The number of apoptotic cells was found to correlated with the ratio of ki-67 positive cell (P = 0.001). There was significant correlation between the extent of apoptosis and the expression of bcl-2 in SCLC, but this relation was not observed in NSCLC. No correlation was found among apoptotic cells or ki-67 positive cells, staging, age and expression of P53. Patients with apoptotic cells greater than 3% and ki-67 greater than 20% possessed shorter survival time than that less than or equal to 3% or 20%, respectively (P = 0.0057, P = 0.002 by log ronk).
Increased apoptotic and proliferous cells are independent prognostic index in lung cancers, predicting shortened survival time of the patients.
观察肺癌细胞凋亡、增殖与相关基因表达之间的关系及其作为预后指标的意义。
采用原位末端标记（ISEL）技术和DNA凝胶电泳检测凋亡细胞，应用免疫组织化学法检测57例肺癌组织中bcl-2、P53和ki-67的表达。
发现凋亡细胞数量与ki-67阳性细胞比例相关（P = 0.001）。小细胞肺癌中凋亡程度与bcl-2表达之间存在显著相关性，但在非小细胞肺癌中未观察到这种关系。凋亡细胞或ki-67阳性细胞、分期、年龄与P53表达之间未发现相关性。凋亡细胞大于3%且ki-67大于20%的患者生存时间分别短于凋亡细胞小于或等于3%或ki-67小于或等于20%的患者（对数秩检验，P = 0.0057，P = 0.002）。
凋亡细胞和增殖细胞增加是肺癌独立的预后指标，提示患者生存时间缩短。
