[Preliminary study on relationship among apoptosis and related gene, prognosis of lung cancer].

OBJECTIVE

To observe the relationship among Lung cancer cell apoptosis, proliferation and related gene expression and its significance as an index of prognosis.

METHOD

In situ end labelling (ISEL) technique and DNA gel electrophoresis were used to detect the apoptotic cell, immunohistochemistry was used to detect the expression of bcl-2, P53 and ki-67 in 57 cases of Lung cancer.

RESULT

The number of apoptotic cells was found to correlated with the ratio of ki-67 positive cell (P = 0.001). There was significant correlation between the extent of apoptosis and the expression of bcl-2 in SCLC, but this relation was not observed in NSCLC. No correlation was found among apoptotic cells or ki-67 positive cells, staging, age and expression of P53. Patients with apoptotic cells greater than 3% and ki-67 greater than 20% possessed shorter survival time than that less than or equal to 3% or 20%, respectively (P = 0.0057, P = 0.002 by log ronk).

CONCLUSIONS

Increased apoptotic and proliferous cells are independent prognostic index in lung cancers, predicting shortened survival time of the patients.